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复苏后类器官活率≥90%
体外维持培养≥10天
稳定维持大肠类器官的经典结构和多谱系细胞类型
经≥10批次复苏培养验证，批次间稳定性强
冻存型iPSC源大肠类器官为快速获取标准化的大肠类器官提供了一种便捷的方法；该大肠类器官由iPSC诱导分化形成，拥有更接近结直肠的特征性结构和细胞谱系；经过高效冻存，每支冻存管中含有500个大肠类器官。无需iPSC诱导分化，复苏后可直接用于下游应用。
| 遗传信息 | |||
| • 类器官类型： | • 来源： | • 性别： | |
| 大肠类器官 | 成纤维细胞 | 女 | |
| • 捐助者状态： | • 年龄： | ||
| 健康 | 20 |
a. Representive bright image of day-45 iColons before and after freezing. Scale bar = 200 μm; b. Viability measure of day-45 iColons before and after freezing. Data is based on n=5 vials of frozen iColons that differentiated from 3 independent batches.
Time-laspe image of day-41 iColons after recovery for 10 days. Scale bar = 200 μm.
