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使用 “人多能干细胞向肺类器官分化系统 (Ca. 3d0010) ” 诱导分化产生
呈现出多级分叉结构与早期肺泡样结构，其中至少包含了肺泡 I-II 型细胞、基底细胞、棒状细胞、 纤毛细胞、肺神经内分泌细胞、巨噬细胞、间充质细胞、成纤维细胞等肺泡及气道生理相关的谱系类型
经过肺纤维化、病毒感染、细菌感染等验证
该研究肺脏发育、呼吸疾病及药物筛选的精准人源工具
“ 即用型 iPSC 源肺类器官 ” 呈现出多级分叉结构与早期肺泡样结构，其中至少包含了肺泡 I-II 型细胞、基底细胞、棒状细胞、 纤毛细胞、肺神经内分泌细胞、巨噬细胞、间充质细胞、成纤维细胞等肺泡及气道生理相关的谱系类型。支持肺脏发育机制的探索、呼吸道疾病的模拟与病理解析、药物开发、以及体内定植等研究。
| 遗传信息 | |||
| • 类器官类型： | • 来源： | • 性别： | |
| 肺类器官 | 成纤维细胞 | 男 | |
| • 捐助者状态： | • 年龄： | ||
| 健康 | 32 |
Characteristics
A day-55 iLung organoids presented airway-like and bronchi-like structure, alveoli-like structures. Scale bar = 500 μm.
Characteristics
Immunofluorescence staining of a day-60 iLung demonstrated the presence of both proximal airway and distal alveolar cell types, including ciliated cells (AcTUB+), basal cells (NGFR+), club cells (CC+), goblet cell (MUC1+), neuroendocrine cells (CHGA+), type I cells (PDPN+), type II cells (SP-B+, SP-C+), resident macrophages (CD68+) and fibroblasts (VIM+). Scale bar = 200 μm.
Application of iLung organoids
An iLung treated with 30-d bleomycin manifested increased dead cells, reduced bronchi/alveoli space, and collapsed architecture. Scale bar = 200 μm.
Compared with control that treated with PBS only, 30-d bleomycin induction resulted in significant ECM accumulation in iLung, identified by the increased staining area of Collagen I and Fibronectin with immunofluorescence. Scale bar = 200 μm.
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