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由 “ 人多能干细胞向小肠类器官分化系统 (Ca. 3d0030) ” 产生
外周间质包裹的极化上皮结构，含有肠上皮细胞、纤毛细胞、潘氏细胞、杯状细胞、神经内分泌细胞、肠隐窝干细胞以及间质细胞
已验证 ＞10 种肠道功能，包括吸收（屏障）、转运、分泌、代谢等
适合作为小肠发育研究、病理解析、肠感染研究、再生研究、以及定植研究的新一代人源化精准工具
“ 即用型 iPSC 小肠类器官 ” 是使用人多能干细胞向小肠类器官分化系统（Ca. 3d0030）诱导分化而成。 “ 即用型 iPSC 小肠类器官 ” 具备腔体、微绒毛、平滑肌等特征性结构；且包含了多种原代小肠所具备的细胞类型，包括肠上皮细胞、神经内分泌细胞、潘氏细胞、杯状细胞、隐窝干细胞、平滑肌细胞、成纤维细胞、肌成纤维细胞等。相应地， “ 即用型 iPSC 小肠类器官 ” 具备譬如吸收（屏障）、转运、分泌、代谢等多种生理相关的功能，适合作为小肠发育研究、病理解析、肠感染研究、再生研究、以及定植研究的新一代人源化精准工具。
| 遗传信息 | |||
| • 类器官类型： | • 来源： | • 性别： | |
| 小肠类器官 | 成纤维细胞 | 男 | |
| • 捐助者状态： | • 年龄： | ||
| 健康 | 32 |
Characteristics
Immunofluorescence demonstrated that day-35 iIntestines were compose of polarized ECAD+ CDX2+ intestinal epithelia and a surrounding VIM+ mesenchyme. Differentiated enterocytes (VIL1+), Paneth cells (LYZ+), goblet cells (MUC2+), enteroendocrine cells (CHGA+) , and intestinal crypt stem cell (LGR5+) were identified within the epithelial tissue. Scale bar = 100 μm.
Characteristics
The efflux of rhodamine 123 (10 μM, a P-gp substrate) into intestine organoids was observed, and this was inhibited by verapamil (50 μM), an inhibitor of P-gp. Scale bar = 200 μm.
Characteristics
The transport of hoechst33342 (10 μM, a specific substrate of BCRP) into intestine organoids was inhibited by its inhibitor Ko143 (50 μM). Scale bar = 200 μm.
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Kwon O, Jung K B, Lee K R, et al. The development of a functional human small intestinal epithelium model for drug absorption[J]. Science advances, 2021, 7(23): eabh1586.
Mithal A, Hume A J, Lindstrom-Vautrin J, et al. Human pluripotent stem cell-derived intestinal organoids model SARS-CoV-2 infection revealing a common epithelial inflammatory response[J]. Stem Cell Reports, 2021, 16(4): 940-953.
