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iPSC向前脑类器官分化试剂盒,3d0080

  • 该分化系统可将人iPSC或ESC高效分化为多谱系前脑类器官

  • 产生的前脑类器官呈现出典型的VZ、SVZ和CP样等结构,含有谷氨酰胺能、多巴胺能和GABA能等神经元,以及星形胶质、少突胶质及小胶质细胞

  • 有试剂均化学成分明确、不含血清

  • 该系统稳定性较好,目前已经通过过4种iPSC的测试


产品规格

  • 1 kit

简介

组分


• iForebrain Neuroectoderm 

  Differentiation Kit

• iForebrain Neural Progenitor 

  Differentiation Kit

• iForebrain Brain Growth and 

  Maintenance Kit

  NE Media l (15mL)   NPC Media I (100mL)   MO Media I (200mL)
  NE Media ll (60mL)   NPC Media II (75mL)   MO Media Il (200mL)






参考文献

  • Madeline,A,Lancaster,et al.Cerebral organoids model human brain development and microcephaly.[J].Nature, 2013.

  • Kadoshima T , Sakaguchi H , Nakano T ,et al.Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex.[J].Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(50):20284-20289.

  • Paca A M , Sloan S A , Clarke L E ,et al.Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture[J].Nature Methods[2025-02-05].

  • Nguyen X Q H N , Song M M , Hadiono C ,et al.Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure[J].Cell, 2016,165(5).

  • Sakaguchi H , Ozaki Y , Ashida T ,et al.Self-Organized Synchronous Calcium Transients in a Cultured Human Neural Network Derived from Cerebral Organoids[J].Stem Cell Reports, 2019, 13(3). 

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