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使用 “人多能干细胞向前脑类器官分化系统 (Ca. 3d0080)” 产生
自发形成 VZ 样、SVZ 样及 CP 样等结构，含有谷氨酰胺能、多巴胺能神经元及少突胶质细胞、星形胶质细胞以及小胶质细胞
已验证前脑自发电生理活动，包括 MEA、自发动作电位及诱发动作电位等电信号的产生
适合作为前脑发育研究，中枢神经系统疾病及药物筛选的精准人源工具
“ 即用型 iPSC 源前脑类器官 ” 是以人诱导多能干细胞 (iPSC, induced pluripotent stem cells) 为起点细胞，基于前脑发育进程的关键分子信号，凭借 iPSC 谱系共分化技术诱导分化形成。iForebrain 具有更接近人真实前脑的特征性结构和细胞谱系构成，为发育研究、病理解析、药物筛选、及再生研究提供新一代人源化精准工具。
| 遗传信息 | |||
| • 类器官类型： | • 来源： | • 性别： | |
| 脑类器官 | 成纤维细胞 | 男 | |
| • 捐助者状态： | • 年龄： | ||
| 健康 | 32 |
Characteristics
Day-65 iForebrains generated using brain differentiation system met dorsal forebrain identity (FOXG1+ NKX2.1+), and displayed human cerebral characteristic structures involving CP (cortical plate, TUJ1+/bright-field), VZ (SOX2+, ventricular zone), and SVZ(TBR2+, subventricular zone). Scale bar = 100 μm.
Characteristics
Immunofluorescence of day-90 iForebrains identified many physiologically relevant neural cell types, including glutaminergic (vGLUT2+), dopaminergic (TH+) and GABAergic neurons (GABA+), as well as astrocytes (GFAP+), oligodendrocytes (Olig2+) and microglia (CD68+).Scale bar = 100 μm.
Activity of iForebrain organoids
MEA (multi-electrode array) recordings from a day-90 iForebrain continuously monitored spontaneous electrophysiological activities.
Patch clamp results
Patch-clamp recordings from a day-89 iForebrain show spontaneous and evoked action potentials.
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