复苏后类器官活率≥90%
体外维持培养≥10天
稳定维持小肠类器官的经典结构和多谱系细胞类型
冻存型iPSC小肠类器官为快速获取标准化的小肠类器官提供了一种便捷的方法;该小肠类器官由iPSC诱导分化形成,拥有更接近十二指肠的特征性结构和细胞谱系;经过高效冻存,每支冻存管中含有100个小肠类器官。无需iPSC诱导分化,复苏后可直接用于下游应用。
遗传信息 | |||
• 类器官类型: | • 来源: | • 性别: | |
小肠类器官 | 成纤维细胞 | 女 | |
• 捐助者状态: | • 年龄: | ||
健康 | 20 |
a. Representive bright image of day-42 iIntestines before and after freezing. Scale bar = 200 μm;b. Viability measure of day-45 iIntestines before and after freezing. Data is based on n=4 vials of frozen iIntestines that differentiated from 4 independent batches.
Time-laspe image of day-43 iIntestines after 7-d recovery. Scale bar = 200 μm .