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由 “人多能干细胞向结肠类器官分化系统 (Cat. 3d0135) ” 产生
细胞：含大肠上皮细胞、杯状细胞、神经内分泌细胞、嗜铬细胞、TA细胞、肠隐窝干细胞等细胞类型
结构：呈复杂3D结构，含极化柱状上皮、空腔、肠隐窝
“ 即用型 iPSC 大肠类器官 ” 是使用人多能干细胞向大肠类器官分化系统（Cat. 3d0135）诱导分化而成。 “ 即用型 iPSC 大肠类器官 ” 具有复杂的 3D 结构；其中包含了多种原代结肠所具备的细胞类型，包括大肠上皮细胞、神经内分泌细胞、杯状细胞、纤毛细胞、肠隐窝干细胞、肌成纤维细胞等。作为新一代的人源化精准体外模型， “ 即用型 iPSC 大肠类器官 ” 可以使研究人员从容不迫地探究发育机制、解析病理机制、发现药物靶点、筛选药毒药效等。
| 遗传信息 | |||
| • 类器官类型： | • 来源： | • 性别： | |
| 大肠类器官 | 成纤维细胞 | 女 | |
| • 捐助者状态： | • 年龄： | ||
| 健康 | 20 |
Characteristics
Immunofluorescent staining showed day-40 iColons were majorly comprised of SATB2+CDX2+ colonic epithelia, in which contain goblet cell (MUC2+), neuroendocrine cells (CHGA+), enterochromaffin cells (5-HT+) and TA cells (EPHB2+). Scale bar = 100 μm.
Assigning Developmental Stages to iColon with KeyGenes Tool
Yokoi F, Deguchi S, Watanabe Y, et al. Establishment of an ulcerative colitis model using colon organoids derived from human induced pluripotent stem cells[J]. Iscience, 2024, 27(10).
Han Y, Duan X, Yang L, et al. Identification of SARS-CoV-2 inhibitors using lung and colonic organoids[J]. Nature, 2021, 589(7841): 270-275.
Mithal A, Hume A J, Lindstrom-Vautrin J, et al. Human pluripotent stem cell-derived intestinal organoids model SARS-CoV-2 infection revealing a common epithelial inflammatory response[J]. Stem Cell Reports, 2021, 16(4): 940-953.
Crespo M, Vilar E, Tsai S Y, et al. Colonic organoids derived from human induced pluripotent stem cells for modeling colorectal cancer and drug testing[J]. Nature medicine, 2017, 23(7): 878-884.
Múnera J O, Sundaram N, Rankin S A, et al. Differentiation of human pluripotent stem cells into colonic organoids via transient activation of BMP signaling[J]. Cell stem cell, 2017, 21(1): 51-64. e6.
